Coding

Part:BBa_K1916000:Design

Designed by: Jonathan Chen   Group: iGEM16_UC_Davis   (2016-10-09)


NpF2164g5 + intein-CBD tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 531
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 531
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 531
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 531
    Illegal NgoMIV site found at 1027
    Illegal AgeI site found at 1117
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.


Source

Protein from cyanobacteria genome (sequence provided with assistance of Nathan Rockwell and Clark Lagarias of the Lagarias Lab, UC Davis).

References